Non-uniform Illumination Correction in Transmission Electron Microscopy
نویسندگان
چکیده
Transmission electron microscopy (TEM) provides resolutions on the order of a nanometer. Hence, it is a critical imaging modality for biomedical analysis at the sub-cellular level. One of the problems associated with TEM images is variations in brightness due to electron imaging defects or non-uniform support films and specimen staining. These variations render image processing operations such as segmentation more difficult. The correction requires estimation of the global illumination field. In this paper, we propose an automatic method for estimating the illumination field using only image intensity gradients. The closed-form solution is very fast to compute.
منابع مشابه
Automatic Correction of Non-uniform Illumination in Transmission Electron Microscopy Images
Transmission electron microscopy (TEM) provides resolutions on the order of a nanometer. Hence, it is a critical imaging modality for biomedical analysis at the cellular level. One of the problems associated with TEM images is variations in brightness due to electron imaging defects or nonuniform support films and specimen staining. These variations render image processing operations such as se...
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